C-RAF KINASE BINDS TO N-TERMINAL DOMAIN OF C-MYC

We have demonstrated that the 50 N-terminal amino acids of c-Myc bind a kinase activity, which phosphorylates Myc in vitro predominantly on Thr(8). We also have shown that c-Raf, a widely known Ser/Thr kinase, involved in the Ras signaling pathway, binds to the same portion of c- Myc in vitro, In addition we were able to precipitate native c-Myc/Raf complex from various cell lysates. Physical interaction of Myc and Ra f may potentially be a part of their well-known functional cooperation . (C) 1997 Federation of European Biochemical Societies.