ISOLATION AND STRUCTURAL IDENTIFICATION OF BIFIDOGENIC GROWTH STIMULATOR PRODUCED BY PROPIONIBACTERIUM-FREUDENREICHII

Authors
MORI H SATO Y TAKETOMO N KAMIYAMA T YOSHIYAMA Y MEGURO S SATO H KANEKO T
Citation
H. Mori et al., ISOLATION AND STRUCTURAL IDENTIFICATION OF BIFIDOGENIC GROWTH STIMULATOR PRODUCED BY PROPIONIBACTERIUM-FREUDENREICHII, Journal of dairy science, 80(9), 1997, pp. 1959-1964
Citations number
18
Categorie Soggetti
Agriculture Dairy & AnumalScience","Food Science & Tenology
Journal title
Journal of dairy science → ACNP
ISSN journal
00220302
Volume
80
Issue
9
Year of publication
1997
Pages
1959 - 1964
Database
ISI
SICI code
0022-0302(1997)80:9<1959:IASIOB>2.0.ZU;2-W
Abstract
A bifidogenic growth stimulator produced by Propionibacterium freudenr eichii was purified, and its chemical structure was determined. We obt ained 7.1 mg of a bifidogenic growth stimulator from 1738 g of lyophil ized P. freudenreichii cells by silica gel column chromatography, Seph adex LH-20 column chromatography, and preparative HPLC. The mass of th e bifidogenic growth stimulator was 217.037 (C11H7NO4) as determined b y high resolution mass spectrometry. Various experimental analyses ind icated that the chemical structure of the bifidogenic growth stimulato r was 2-amino-3-carboxy-1,4-naphthoquinone.