PRELIMINARY-STUDY OF OVARIAN ACTIVITY IN FILLIES TREATED WITH A GNRH VACCINE

Objective To investigate the effects of two doses (200 and 400 mg) of a water-soluble gonadotrophin-releasing hormone vaccine on the ovarian activity of 2-year-old fillies. Design A controlled vaccination dose rate experiment. Animals Six 2-year-old Australian Stock Horse fillies were randomly allocated to three treatment groups: unvaccinated contr ols, those receiving 200 mg of the vaccine and those receiving 400 mg of the vaccine. Results Ovarian activity of the treated fillies was su ppressed at the peak of the breeding season while that of the untreate d controls continued normally. The control fillies displayed oestrous activity and behaviour. Suppression of ovarian activity occurred for 2 5 and 30 weeks in the 200 and 400 mg groups, respectively. These diffe rences were not significant. Ovarian activity ceased 2 to 3 weeks afte r primary vaccination. Antibody titres were low (330) until after the booster immunisation when they rapidly peaked at 22,000 and 28,000 in the 200 mg and 400 mg groups, respectively. Plasma progesterone concen trations of the treated fillies remained below 3.18 nmol/L while GnRH was suppressed. The vaccine had no significant effect on plasma andros tenedione concentrations. Recovery from the effect of the vaccine was associated with development of ovarian follicles, normal oestrous beha viour and ovulation. Three of the four treated fillies and one of the controls conceived during the next breeding season and foaled normally . All the treated fillies conceived and produced normal foals in the f ollowing two breeding seasons. Conclusion Both dose rates suppressed o varian function and prevented oestrous behaviour. These effects were r eversible and the subsequent fertility of the vaccinated fillies was n ormal.