DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS BY USEOF A RAPID, SIMPLE, AND SPECIFIC RNA RNA MISMATCH ASSAY/

Citation
Ka. Nash et al., DETECTION OF RIFAMPIN RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS BY USEOF A RAPID, SIMPLE, AND SPECIFIC RNA RNA MISMATCH ASSAY/, The Journal of infectious diseases, 176(2), 1997, pp. 533-536
Citations number
14
Categorie Soggetti
Infectious Diseases
ISSN journal
00221899
Volume
176
Issue
2
Year of publication
1997
Pages
533 - 536
Database
ISI
SICI code
0022-1899(1997)176:2<533:DORRIM>2.0.ZU;2-Z
Abstract
An adaption of an RNA/RNA duplex, base pair-mismatch assay is capable of detecting rifampin resistance in Mycobacterium tuberculosis, The sp ecificity and sensitivity of the mismatch assay in detecting rifampin resistance were 100% and 96%, respectively, when tested against 46 rif ampin-resistant and rifampin-susceptible strains of M. tuberculosis, B y use of a range of mycobacterial and nonmycobacterial prokaryote path ogens, the mismatch assay was shown to be specific for M. tuberculosis and Mycobacterium bovis. The assay is cost-effective compared with DN A sequencing and other molecular methods and is simple to perform and interpret, Furthermore, the assay can return a result within 24 h afte r receipt of an isolated organism and potentially can be used directly with smear-positive specimens.