THE INCUBATION PERIOD AND REACTION OF ONE TYPE OF ANTIBODY TO CD3 ANTIGEN IN LYMPHOCYTE SMEARS OF DIFFERENT ANIMALS

Authors
LEVKUTOVA M REVAJOVA V
Citation
M. Levkutova et V. Revajova, THE INCUBATION PERIOD AND REACTION OF ONE TYPE OF ANTIBODY TO CD3 ANTIGEN IN LYMPHOCYTE SMEARS OF DIFFERENT ANIMALS, Acta veterinaria, 47(4), 1997, pp. 203-206
Citations number
8
Categorie Soggetti
Veterinary Sciences
Journal title
Acta veterinaria → ACNP
ISSN journal
05678315
Volume
47
Issue
4
Year of publication
1997
Pages
203 - 206
Database
ISI
SICI code
0567-8315(1997)47:4<203:TIPARO>2.0.ZU;2-Z
Abstract
Immunocytochemical detection of CD3 cells in lymphocyte smears of seve ral animals species (dog, pig, sheep, and cattle) is described. Differ ent incubation times with polyclonal antibodies were used (30 min, 90 min, 6 h, and 18 h). CD3 lymphocytes showed an intense immune reaction in the cytoplasmic membrane of these animal cells. An incubation time of 90 min is suitable for using that antiserum in lymphocyte smears.