Among five pigments detected in bambara groundnut (Vigna subterranea),
three major anthocyanins were isolated by combined column chromatogra
phy on Amberlite XAD-7, Sephadex LH-20, and RP-18 silica gel and by pr
eparative thin-layer chromatography on silica gel. They were identifie
d as delphinidin 3-O-beta-glucoside, petunidin 3-O-beta-glucoside, and
malvidin 3-O-beta-glucoside on the basis of chromatographic data and
UV-vis, H-1-NMR, and mass spectrometry.