MICROBIAL TRANSFORMATIONS OF THE FUNGICIDE CYPRODINIL (CGA-219417)

A collection of 12 microbial cultures, known to contain cytochrome P-4 50 monooxygenase or other degradative enzymes, was screened for their ability to degrade the Novartis Crop Protection Inc. developmental fun gicide cyprodinil (CGA-219417; 4-cyclopropyl-6-methyl-N-phenyl-2-pyrim idinamine). Ten of the 12 cultures produced a monohydroxylated metabol ite in yields ranging from 1.2 to 35.6%. The filamentous fungus, Beauv eria bassiana ATCC 7159, produced a methoxylated glycoside of the mono hydroxylated metabolite with a yield of 80%. Dihydroxylated metabolite s and a molecular cleavage product, 4-cyclopropyl-6-methyl-2-pyrimidam ine, were also detected in certain cultures. The overall results of th e study indicated that cyprodinil was readily metabolized by a variety of microbial species. Metabolites generated by these cultures can pot entially be used as analytical reference standards to support animal, plant, and soil metabolism studies.