A. Abad et al., HAPTEN SYNTHESIS AND PRODUCTION OF MONOCLONAL-ANTIBODIES TO DDT AND RELATED-COMPOUNDS, Journal of agricultural and food chemistry, 45(9), 1997, pp. 3694-3702
This work describes the production and characterization of monoclonal
antibodies (MAbs) to the organochlorine insecticide DDT and their inco
rporation into several ELISA. configurations. A collection of DDT hapt
ens was synthesized by introducing appropriate spacers at two sites of
the analyte molecular structure. From mice immunized with hapten-prot
ein conjugates, MAbs with I-50 values to p,p'-DDT in the 2-11 nM range
in homologous conjugate-coated assays were obtained. According to the
ir cross-reactivity pattern with DDT isomers and metabolites, MAbs can
be classified as class-specific or DDT-specific antibodies. Both type
s of MAbs were obtained from mice immunized with the same hapten-prote
in conjugate simply by applying a different selection criterion in the
screening of fusion supernatants. These immunoassays are potentially
very valuable analytical tools for the rapid and sensitive determinati
on of DDT and congeners in food and the environment and for monitoring
human exposure to these ubiquitous and toxic compounds.