A total of 220 microbiology departments in 205 general hospitals, 12 u
niversity hospitals, and 3 military hospitals were surveyed by questio
nnaire during the period March-May 1996 in order to evaluate the statu
s of diagnostic anaerobic bacteriology in Italy. Responses were receiv
ed from 47 laboratories (21.3%). The number of beds in the responding
facilities varied widely (129-2,200), as did the number of specimens t
ested for anaerobic bacteriology (50-29,900). In most cases (94%), the
microbiologist made the decision to culture and to proceed to further
identification of isolates (mainly by commercial systems), depending
on the adequacy of samples. Antibiotic susceptibility testing was perf
ormed in 52.5% of laboratories surveyed. Although it is difficult to d
raw relevant conclusions owing to the poor response rate, it seems tha
t the condition of anaerobic bacteriology in Italy is far from satisfa
ctory. Among major limitations are low interest in the field, lack of
standardization of procedures, poor utilization of reference centers,
and limited technological resources.