The mycelia of Aspergillus kawachii were treated with colchicine solut
ion for the purpose of autoployploidization. When the mycelia were tre
ated with 0.1% (w/v) colchicine solution for 7 days, huge autopolyploi
d nuclei called hyperpolyploid nuclei were produced in the mycelia. Wh
en such mycelia were treated with Benlate, the acid hyper-producing st
rains were isolated at a frequency of 3.0% among conidia on fan-shaped
sectors. it was concluded that the method may contribute to isolating
acid hyper-producing strains of this fungus.