PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY AND SEQUENCING AS AN ALTERNATIVE TO SEROLOGY FOR M-ANTIGEN TYPING OF STREPTOCOCCUS-PYOGENES

Authors
SAUNDERS NA HALLAS G GAWORZEWSKA ET METHERELL L EFSTRATIOU A HOOKEY JV GEORGE RC
Citation
Na. Saunders et al., PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY AND SEQUENCING AS AN ALTERNATIVE TO SEROLOGY FOR M-ANTIGEN TYPING OF STREPTOCOCCUS-PYOGENES, Journal of clinical microbiology, 35(10), 1997, pp. 2689-2691
Citations number
12
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
10
Year of publication
1997
Pages
2689 - 2691
Database
ISI
SICI code
0095-1137(1997)35:10<2689:PIASAA>2.0.ZU;2-L
Abstract
A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were de termined, and in 144 of 149 cases (97%) the results were congruous. St rains of types that were not included in the panel of capture probes w ere emm genotyped by sequencing.