DETERMINATION OF TRITON X-100 IN INFLUENZA VACCINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS

Authors
Citation
K. Heinig et C. Vogt, DETERMINATION OF TRITON X-100 IN INFLUENZA VACCINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS, Fresenius' journal of analytical chemistry, 359(2), 1997, pp. 202-206
Citations number
14
Categorie Soggetti
Chemistry Analytical
ISSN journal
09370633
Volume
359
Issue
2
Year of publication
1997
Pages
202 - 206
Database
ISI
SICI code
0937-0633(1997)359:2<202:DOTXII>2.0.ZU;2-I
Abstract
Triton X-100 is applied to influenza vaccines at different stages of t he manufacturing process to prevent aggregation and precipitation of b iomolecules. Furthermore it is used to disintegrate the virus particle s in split vaccine and to guarantee the homogeneity during production and utilisation. The final concentration of Triton X-100 has to be det ermined because the concentration changes in manufacturing process. Th e determination of the total amount of Triton X-100 as well as the sep aration of its ethylene oxide oligomers was possible with high-perform ance liquid chromatography (HPLC) and capillary electrophoresis (CE). In HPLC a change of the column and eluent was necessary. in CE differe nt electrolytes were used for the various separation effects. The HPLC method for the analysis of total Triton was preferred for the quantif ication of Triton X-100 in influenza vaccine because of better lineari ty, reproducibility and detection sensitivity compared to CE. In the e nd products an average concentration of 0.117 mg/mL was found.