K. Heinig et C. Vogt, DETERMINATION OF TRITON X-100 IN INFLUENZA VACCINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS, Fresenius' journal of analytical chemistry, 359(2), 1997, pp. 202-206
Triton X-100 is applied to influenza vaccines at different stages of t
he manufacturing process to prevent aggregation and precipitation of b
iomolecules. Furthermore it is used to disintegrate the virus particle
s in split vaccine and to guarantee the homogeneity during production
and utilisation. The final concentration of Triton X-100 has to be det
ermined because the concentration changes in manufacturing process. Th
e determination of the total amount of Triton X-100 as well as the sep
aration of its ethylene oxide oligomers was possible with high-perform
ance liquid chromatography (HPLC) and capillary electrophoresis (CE).
In HPLC a change of the column and eluent was necessary. in CE differe
nt electrolytes were used for the various separation effects. The HPLC
method for the analysis of total Triton was preferred for the quantif
ication of Triton X-100 in influenza vaccine because of better lineari
ty, reproducibility and detection sensitivity compared to CE. In the e
nd products an average concentration of 0.117 mg/mL was found.