DEVELOPMENT AND VALIDATION OF A CAPILLARY ZONE ELECTROPHORESIS METHODFOR THE DETERMINATION OF ATROPINE, HOMATROPINE AND SCOPOLAMINE IN OPHTHALMIC SOLUTIONS

A capillary zone electrophoresis method is described for the simultane ous determination of atropine, homatropine and scopolamine. Successful results were obtained after optimization of the electrophoretic param eters such as buffer composition and pH. The best separation was achie ved using a 100 mM Tris-phosphate running buffer at pH 7. The validati on data proved that the method had the requisite selectivity, sensitiv ity, reproducibility and linearity to be used for the assay of these c ompounds in pharmaceutical formulations. Dosage of the separate drugs in ophthalmic preparations is also presented. (C) 1997 Elsevier Scienc e B.V.