Kh. Lee et al., GENETIC-POLYMORPHISM OF CYTOCHROME P-4502E1 AND MITOCHONDRIAL ALDEHYDE DEHYDROGENASE IN A KOREAN POPULATION, Alcoholism, clinical and experimental research, 21(6), 1997, pp. 953-956
Mitochondrial aldehyde dehydrogenase (ALDH2) is mainly responsible for
the oxidation of acetaldehyde generated during alcohol oxidation in v
ivo. Cytochrome P-4502E1 (CYP2E1), a liver microsomal enzyme, also met
abolizes acetaldehyde and ethanol. Genetic polymorphism of ALDH2 and C
YP2E1 was investigated among 481 Korean adults. A new restriction frag
ment-length polymorphism method was developed to determine the genotyp
e of the ALDH2 alleles. This method proved to be simpler and faster th
an the hybridization method using allele-specific oligonucleotide prob
es and polymerase chain reaction-directed mutagenesis. The allele freq
uencies of ALDH2(1) and ALDH2(2) were 0.840 and 0.160, respectively. T
his allele frequency of ALDH2(2) is less than in Japanese people. Gene
tic polymorphism of CYP2E1 was investigated using polymerase chain rea
ction and restriction fragment-length polymorphism. The estimated alle
le frequencies for c1 and c2 were 0.808 and 0.192.