THE REGULATION OF STREPTOCOCCUS-MUTANS GLUCAN-BINDING PROTEIN-A EXPRESSION

Citation
Ja. Banas et al., THE REGULATION OF STREPTOCOCCUS-MUTANS GLUCAN-BINDING PROTEIN-A EXPRESSION, FEMS microbiology letters, 154(2), 1997, pp. 289-292
Citations number
10
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03781097
Volume
154
Issue
2
Year of publication
1997
Pages
289 - 292
Database
ISI
SICI code
0378-1097(1997)154:2<289:TROSGP>2.0.ZU;2-#
Abstract
The S. mutans GBP-A is hypothesized to be constitutively expressed and to contribute to the sucrose-dependent colonization of S. mutans. To investigate GBP-A expression, a reporter gene encoding chloramphenicol acetyltransferase (CAT) was placed downstream of the gbpA promoter an d CAT activity was measured under conditions that would be associated with the sucrose-dependent colonization of S. mutans. Expression of GB P-A was optimal under anaerobiosis and neutral pH conditions, and corr elated with optimal growth. The addition of sucrose to the growth medi um did not elevate the expression of GBP-A.