CLONING OF THE ACEF GENE ENCODING THE PHOSPHOMANNOSE ISOMERASE AND GDP-MANNOSE PYROPHOSPHORYLASE ACTIVITIES INVOLVED IN ACETAN BIOSYNTHESISIN ACETOBACTER-XYLINUM

Authors
GRIFFIN AM POELWIJK ES MORRIS VJ GASSON MJ
Citation
Am. Griffin et al., CLONING OF THE ACEF GENE ENCODING THE PHOSPHOMANNOSE ISOMERASE AND GDP-MANNOSE PYROPHOSPHORYLASE ACTIVITIES INVOLVED IN ACETAN BIOSYNTHESISIN ACETOBACTER-XYLINUM, FEMS microbiology letters, 154(2), 1997, pp. 389-396
Citations number
27
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
154
Issue
2
Year of publication
1997
Pages
389 - 396
Database
ISI
SICI code
0378-1097(1997)154:2<389:COTAGE>2.0.ZU;2-W
Abstract
The aceF gene from Acetobacter xylimum was identified and cloned from a genomic DNA library. The complete DNA sequence was determined and co mputer analysis of the translated gene sequence revealed homology with the deduced amino acid sequence of xanB from Xanthomonas campestris. Therefore aceF is likely to encode a bifunctional enzyme with mannose- 6-phosphate isomerase (PMI) and GDP-mannose pyrophosphorylase (GMP) ac tivities. PMI and GMP activities were detected in strains of Escherich ia coil expressing the cloned aceF gene.