THE INHIBITION OF GLUTAMATE RACEMASE BY D-N-HYDROXYGLUTAMATE

Authors
GLAVAS S TANNER ME
Citation
S. Glavas et Me. Tanner, THE INHIBITION OF GLUTAMATE RACEMASE BY D-N-HYDROXYGLUTAMATE, Bioorganic & medicinal chemistry letters, 7(17), 1997, pp. 2265-2270
Citations number
30
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
7
Issue
17
Year of publication
1997
Pages
2265 - 2270
Database
ISI
SICI code
0960-894X(1997)7:17<2265:TIOGRB>2.0.ZU;2-N
Abstract
We report that D-N-hydroxyglutamate is ii competitive inhibitor of glu tamate racemase (K-I=56 mu M). The compound acts as an alternate subst rate and is converted into a-ketoglutarate and ammonia (K-M=57 mu M, k (cat)/K-M=3.2x10(3) M(-1)sec(-1)), An imine intermediate is likely the species causing the inhibition. L-N-hydroxyglutamate is a weak inhibi tor and is slowly converted to alpha-ketoglutarate (k(cat)/K-M=30 M(-1 )sec(-1)). (C) 1997 Elsevier Science Ltd.