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Results:
1-19
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Results: 19
Authors:
Wong, E
Sim, J
Sim, TS
Citation: E. Wong et al., Construction of Streptomyces jumonjinensis isopenicillin N synthase doublemutants, Ser119Ala/Glu120Gly and Thr308Ala/Thr309Val, overcomes insoluble expression in Escherichia coli, J MOL CAT B, 13(1-3), 2001, pp. 17-25
Authors:
Loke, P
Wee, J
Seah, KI
Sim, TS
Citation: P. Loke et al., PCR-mediated screening and cloning of a malate synthase genefrom Streptomyces griseus NCIMB 9001, WORLD J MIC, 17(6), 2001, pp. 645-649
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Replacement of tyrosine-197 and the corresponding tyrosine-195 to isoleucine in Cephalosporium acremonium and Streptomyces clavuligerus isopenicillinN synthase, Z NATURFO C, 56(9-10), 2001, pp. 806-809
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, A comparison of three site-directed mutagenesis kits, Z NATURFO C, 56(9-10), 2001, pp. 810-813
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Site-directed mutagenesis of proline-285 to leucine in Cephalosporium acremonium isopenicillin N synthase affects catalysis and increases soluble expression at higher temperatures, Z NATURFO C, 56(5-6), 2001, pp. 413-415
Authors:
Sim, TS
Loke, P
Lee, MA
Singh, M
Flotow, H
Citation: Ts. Sim et al., Cloning and sequence characterisation of falcipain-2 from Plasmodium falciparam Gombak A strain (Malaysia), PARASIT RES, 87(9), 2001, pp. 683-686
Authors:
Goh, LL
Loke, P
Sim, TS
Citation: Ll. Goh et al., Replacement of arginine-171 and aspartate-453 in Streptomyces coelicolor malate synthase A by site-directed mutagenesis inactivates the enzyme, APPL MICR B, 57(3), 2001, pp. 363-367
Authors:
Sim, J
Sim, TS
Citation: J. Sim et Ts. Sim, In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase, ENZYME MICR, 29(4-5), 2001, pp. 240-245
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Mutational analysis of conserved glycines 42 and 256 in Cephalosporium acremonium isopenicillin N synthase, CAN J MICRO, 47(10), 2001, pp. 961-964
Authors:
Chin, HS
Sim, J
Sim, TS
Citation: Hs. Chin et al., Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion, BIOC BIOP R, 287(2), 2001, pp. 507-513
Authors:
Wong, E
Sim, J
Sim, TS
Citation: E. Wong et al., The invariant F283 and its strategic position in the hydrophobic cleft of Streptomyces jumonjinensis isopenicillin N synthase active site are functionally important, BIOC BIOP R, 283(3), 2001, pp. 621-626
Authors:
Sim, J
Sim, TS
Citation: J. Sim et Ts. Sim, Mutational evidence supporting the involvement of tripartite residues His183, Asp185, and His243 in Streptomyces clavuligerus deacetoxycephalosporin C synthase for catalysis, BIOS BIOT B, 64(4), 2000, pp. 828-832
Authors:
Sim, TS
Loke, P
Citation: Ts. Sim et P. Loke, Molecular studies on isopenicillin N synthases, APPL MICR B, 54(1), 2000, pp. 1-8
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Mutational analysis of tyrosine-191 in the catalysis of Cephalosporium acremonium isopenicillin N synthase, J BIOCHEM, 127(4), 2000, pp. 585-589
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Molecular cloning, heterologous expression, and functional characterisation of a malate synthase gene from Streptomyces coelicolor A3(2), CAN J MICRO, 46(8), 2000, pp. 764-769
Authors:
Loke, P
Ng, CP
Sim, TS
Citation: P. Loke et al., PCR cloning, heterologous expression, and characterization of isopenicillin N synthase from Streptomyces lipmanii NRRL 3584, CAN J MICRO, 46(2), 2000, pp. 166-170
Authors:
Sim, J
Sim, TS
Citation: J. Sim et Ts. Sim, Amino acid substitutions affecting protein solubility: high level expression of Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli, J MOL CAT B, 6(3), 1999, pp. 133-143
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Site-directed mutagenesis of arginine-89 supports the role of its guanidino side-chain in substrate binding by Cephalosporium acremonium isopenicillin N synthase, FEMS MICROB, 179(2), 1999, pp. 423-429
Authors:
Loke, P
Sim, TS
Citation: P. Loke et Ts. Sim, Glutamine-230 influences enzyme solubility but not catalysis in Streptomyces clavuligerus isopenicillin N synthase, FEMS MICROB, 173(2), 1999, pp. 439-443
Risultati:
1-19
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