Poultry litter is a mixture of excreta, feed, feathers, and bedding ma
terial. Poultry litter is useful as a fertilizer due to the presence o
f nitrogen phosphorus, and potassium. Litter is commonly used for grow
ing corn, soybeans, potatoes, tomatoes, leafy vegetables, and cover cr
ops. The aqueous leachate of poultry Litter has been shown to exhibit
toxicity to a variety of indicator organisms. The objective of this re
search was to identify classes of toxicants in the litter aqueous leac
hate. The aqueous extract of poultry Litter was subjected to toxicity
identification tests approved by the United States Environmental Prote
ction Agency that use Ceriodaphnia dubia as the test organism. Tests p
erformed included pH adjustment, filtration, aeration, C-18 solid-phas
e extraction, EDTA addition, sodium thiosulfate addition, and extracti
on through zeolite. Zeolite extraction, filtration at low pH, and aera
tion at low pH reduced overall toxicity by 68, 20, and 22%, respective
ly. The major sources of toxicity appear to be ammonia and anionic org
anic compounds. Toxicity was apparently not due to the presence of hea
vy metals or oxidants.