Recent reports suggest that dextrins produced by anti-staling alpha-am
ylases are directly involved in retarding the staling process. We repo
rt experiments that test this hypothesis using a new method of dextrin
extraction at 90 degrees C in the presence of papain. Various dextrin
s were added to standard bread and extracted under a variety of condit
ions. No change in staling rate was observed, but, in most cases, ther
e was little difference between the level of dextrins extracted from b
aked loaves and that from the control leaves, confirming that added de
xtrins are hydrolysed by native flour amylases. Adding a bacterial alp
ha-amylase of intermediate temperature stability (Novamyl) or a fungal
glucoamylase of intermediate temperature stability (GA300N) or alpha-
amylase plus glucoamylase to the dough, produced leaves with different
dextrin profiles. Measured staling rates did not relate to the presen
ce of dextrins in a specific size class. It appears that dextrins pres
ent in an amylase-treated loaf are symptomatic of a modification to th
e starch that retards staling, but are not the direct cause of the ant
i-staling effect. (C) 1997 Academic Press Limited.