VARIANTS OF TRYPSIN-INHIBITORS IN CULTIVATED AND WILD BARLEY AND ANALYSIS OF THEIR ANTITRYPSIN ACTIVITY

Authors
Citation
Mp. Ladogina, VARIANTS OF TRYPSIN-INHIBITORS IN CULTIVATED AND WILD BARLEY AND ANALYSIS OF THEIR ANTITRYPSIN ACTIVITY, Journal of cereal science, 26(2), 1997, pp. 265-270
Citations number
16
Categorie Soggetti
Food Science & Tenology
Journal title
ISSN journal
07335210
Volume
26
Issue
2
Year of publication
1997
Pages
265 - 270
Database
ISI
SICI code
0733-5210(1997)26:2<265:VOTICA>2.0.ZU;2-K
Abstract
Five variants of trypsin inhibitors were found in 80 cultivated and 20 wild spring barley accessions studied by native alkaline electrophore sis with subsequent development of antitrypsin activity: Three of the variants corresponded to the previously described BTI-CMe1, CMe2, and CMe3 proteins. The other two were new and were designated as BTI-CMe4 and BTI-CMe5. CMe4 occurred in both cultivated and wild barley, CMe5 w as found only in wild barley. Out of 12 cultivars studied, cultivars w ith CMe4 variant were characterised by the lowest activity. Besides th e main trypsin inhibitor, an additional zone of antitrypsin activity w as found in each BTI-CMe variant of cultivated barley. It was shown th at BTI content varied significantly among varieties. These differences were associated with BTI variants and did not correlate with the leve l of soluble protein in grain. (C) 1997 Academic Press Limited.