OXIDATIVE-DEGRADATION AND DETOXIFICATION OF MYCOTOXINS USING A NOVEL SOURCE OF OZONE

Citation
Ks. Mckenzie et al., OXIDATIVE-DEGRADATION AND DETOXIFICATION OF MYCOTOXINS USING A NOVEL SOURCE OF OZONE, Food and chemical toxicology, 35(8), 1997, pp. 807-820
Citations number
65
Categorie Soggetti
Toxicology,"Food Science & Tenology
ISSN journal
02786915
Volume
35
Issue
8
Year of publication
1997
Pages
807 - 820
Database
ISI
SICI code
0278-6915(1997)35:8<807:OADOMU>2.0.ZU;2-H
Abstract
Practical methods to degrade mycotoxins using ozone gas (O-3) have bee n limited due to low O-3 production capabilities of conventional syste ms and their associated costs. Recent advances in electrochemistry (i. e. proton-exchange membrane and electrolysis technologies) have made a vailable a novel and continuous source of O-3 gas up to 20% by weight. It is possible that the rapid delivery of high concentrations of O-3 will result in mycotoxin degradation in contaminated grains-with minim al destruction of nutrients. The major objectives of this study were t o investigate the degradation and detoxification of common mycotoxins in the presence of high concentrations of O-3 In this study, aqueous e quimolar (32 mu M) solutions of aflatoxins B-1 (AfB(1)), B-2 (AfB(2)), G(1) (AfG(1)), G(2) (AfG(2)), cyclopiazonic acid (CPA), fumonisin B-1 (FB1), ochratoxin A (OA), patulin, secalonic acid D (SAD) and zearale none (ZEN) were treated with 2, 10 and/or 20 weight% O-3 over a period of 5.0 min and analysed by HPLC. Results indicated that AfB(1) and Af G(1) were rapidly degraded using 2% O-3, while AfB(2) and AfG(2) were more resistant to oxidation and required higher levels of O-3 (20%) fo r rapid degradation. In other studies, patulin, CPA, OA, SAD and ZEN w ere degraded at 15 sec, with no by-products detectable by HPLC. Additi onally, the toxicity of these compounds (measured by a mycotoxin-sensi tive bioassay) was significantly decreased following treatment with O- 3 for 15 sec. In another study, FB1 (following reaction with O-3) was rapidly degraded at 15 sec, with the formation of new products. One of these appeared to be a 3-keto derivative of FB1. Importantly, degrada tion of FB1 did not correlate with detoxification, since FB1 solutions treated with O-3 were still positive in two bioassay systems. (C) 199 7 Elsevier Science Ltd.