DETERMINATION OF TYLOSIN IN MILK BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Authors
USLEBER E STRAKA M MARTLBAUER E
Citation
E. Usleber et al., DETERMINATION OF TYLOSIN IN MILK BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY, Archiv für Lebensmittelhygiene, 48(4), 1997, pp. 75-76
Citations number
3
Categorie Soggetti
Public, Environmental & Occupation Heath","Food Science & Tenology
Journal title
Archiv für Lebensmittelhygiene → ACNP
ISSN journal
0003925X
Volume
48
Issue
4
Year of publication
1997
Pages
75 - 76
Database
ISI
SICI code
0003-925X(1997)48:4<75:DOTIMB>2.0.ZU;2-D
Abstract
A method for the determination of tylosin in milk is reported. Milk sa mples were defatted, diluted with water and purified using RP18 solid phase extraction columns. Determination was achieved by reversed-phase liquid chromatography and UV detection at 287 nm. Peak identity was c hecked by recording the UV spectrum. Detection limit for tylosin in mi lk was 12.5-15 ng/ml. Recoveries for tylosin from spiked milk samples (concentration range: 25-100 ng/ml) were 81.7-87.6%, with coefficients of variation of 7.32-17.1%.