B. Becker et Wh. Holzapfel, MICROBIOLOGICAL RISK OF PREPACKED SPROUTS AND MEASURES TO REDUCE TOTAL COUNTS, Archiv für Lebensmittelhygiene, 48(4), 1997, pp. 81-84
There is a nutritional trend towards light, low calorie and healthy fo
od including mainly fresh vegetables and sprouts. Sprouts, a perishabl
e, susceptible and usually highly contaminated produce, is preferably
consumed uncooked. This involves the risk of food intoxication. 48 com
mercial prepacked sprouts (alfalfa, lentils, wheat, peas, raphanus, su
nflowers, mungbeans and red radish) were studied microbiologically for
total counts, enterobacteriae and pseudomonads, lactic acid bacteria,
yeasts and moulds. Total counts under aerobic conditions were 1.0 x 1
0(5) to 2.2 x 10(9)/g. More than 80 % of the samples contained 10(8) t
o 10(9) microorganisms/g. Two samples of mungbean-and one of alfalfa s
prouts contained 2.6 x 10(5) and 4 x 10(5) Bacillus cereus/g, respecti
vely, The sprouts investigated contained no salmonallae, but in 5 case
s the non-pathogenic species Listeria innocua. Enterobacteriaceae and
pseudomonads were the dominant groups with counts between 1.0 x 10(4)
and 5.0 x 10(7) cfu/g. The lactic acid bacteria counts varied from 1.0
x 10(2) to 5.0 x 10(7) cfu/g, while yeasts and moulds counts ranged f
rom 1.0 x 10(2) to 8.0 x 10(6) cfu/g. Thorough washing of the sprouts
in water was not sufficient for decontamination; it reduced total coun
ts by less than one order of magnitude. Rinsing in 1 % and 2 % lactic
and acetic acid, respectively, reduced total counts by 96 %. Blanching
of mungbean sprouts for 1 min at 90 degrees C was the only method to
reduce counts sufficiently to 7.4 x 10(2) cfu/g and still preserve vit
amin C.