PURIFICATION OF SOYBEAN LIPOXYGENASE ISOENZYME-1 AND CHARACTERIZATIONOF ITS INHIBITION BY 13-HYDROPEROXIDES

Authors
FAUCONNIER ML MARLIER M
Citation
Ml. Fauconnier et M. Marlier, PURIFICATION OF SOYBEAN LIPOXYGENASE ISOENZYME-1 AND CHARACTERIZATIONOF ITS INHIBITION BY 13-HYDROPEROXIDES, Grasas y aceites, 47(4), 1996, pp. 242-246
Citations number
16
Categorie Soggetti
Food Science & Tenology","Chemistry Applied
Journal title
Grasas y aceites → ACNP
ISSN journal
00173495
Volume
47
Issue
4
Year of publication
1996
Pages
242 - 246
Database
ISI
SICI code
0017-3495(1996)47:4<242:POSLIA>2.0.ZU;2-1
Abstract
Purification of soybean lipoxygenase isoenzyme-l was reinvestigated fu rnishing a new procedure. Different parameters influencing the hydrope roxide production were studied. Our study focused particularly on the enzyme activity inhibition by the reaction products. Dixon plots revea led competitive inhibition with Ki value of 0.45 and 1.5 mM respective ly for 13-linoleic and 13-linolenic acid hydroperoxides.