SIMULTANEOUS MONITORING OF GLUTATHIONE AND MAJOR PROTEINS IN SINGLE ERYTHROCYTES

To gain more information from single-cell analysis, multiple detection schemes can be implemented in capillary electrophoresis. Red blood ce lls pre-treated with monobromobimane allow fluorescent tags to be atta ched to various thiol-containing compounds. Both derivatized glutathio ne and derivatized hemoglobin-A(0) can be detected by observing emissi on at 495 nm when 275 nm excitation is used. At the same time, a secon d emission channel at 350 nm can be used to monitor the native fluores cence from carbonic anhydrase and from hemoglobin. Cross-correlation o f the intracellular amounts of these provides additional insight into the cell-aging process.