FRACTIONATION OF PHENOLASE FROM GREEN TABLE OLIVES (ASCOLANA-TENERA VAR) BY IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY

Authors
SCIANCALEPORE V DESTEFANO G
Citation
V. Sciancalepore et G. Destefano, FRACTIONATION OF PHENOLASE FROM GREEN TABLE OLIVES (ASCOLANA-TENERA VAR) BY IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY, Grasas y aceites, 46(4-5), 1995, pp. 251-254
Citations number
28
Categorie Soggetti
Food Science & Tenology","Chemistry Applied
Journal title
Grasas y aceites → ACNP
ISSN journal
00173495
Volume
46
Issue
4-5
Year of publication
1995
Pages
251 - 254
Database
ISI
SICI code
0017-3495(1995)46:4-5<251:FOPFGT>2.0.ZU;2-E
Abstract
Phenolase from green table olives (Ascolana tenera var.) was fractiona ted by immobilized copper affinity chromatography. Four chromatographi c fractions with high specific activities were obtained. The highest a ctivity was found for the fraction eluted with L-histidine buffer. The four active fractions were characterized by their response to both sp ecificity and concentration of the substrate.