CHARACTERIZATION OF A NOVEL HIGH M(R) GLUTENIN SUBUNIT ENCODED BY CHROMOSOME 1D OF BREAD WHEAT

A high M(r) subunit of wheat glutenin (1Dx2.2), having an unusually h igh M(r) (approximately 110 000), was purified using a combination of preparative isoelectric focusing and reversed-phase high performance l iquid chromatography. Comparison of its N-terminal sequence and amino acid composition with those of the allelic subunit 1Dx2 indicate that its greater M(r) could be due to the presence of a larger central repe titive domain. Spectrofluorimetric and circular dichroism analyses sug gest that an increase in the length of this domain does not affect the conformational properties of the subunit. (C) 1996 Academic Press Lim ited