A total of 302 lactic acid bacteria (LAB) isolates were obtained from
samples taken at various stages during manufacture and ripening of Arz
ua cheese (a traditional) raw cows' milk cheese from northwest Spain).
Of the 302 isolates, 121 were identified as Lactococcus lactis subsp.
lactis, 49 as Lactobacillus casei, six as Lb. plantarum, 49 as Leucon
ostoc mesenteroides, 22 as Ln. paramesenteroides and one as Ln. lactis
. Forty-six isolates were identified as enterocci. Between the start o
f mnaufacture and day 15 of ripening, Lactococcus isolates were the mo
st numerous,. towards the end of ripening, lactococci and lactobacilli
isolates were about equally frequent. The majority of the lactococci
isolates had weak acidifying and slow milk-coagulating activities. Mos
t lactobacilli were weakly proteolytic, and unconventional biochemical
characteristics were very frequent among these isolates. Of the leuco
nostocs isolates, only that identified as Ln. lactis produced acetoin
from citrate.