DETERMINATION OF PLATINUM METALS COMPLEXES WITH 6-METHYL-2-BENZOTHIAZOLYLAZO)-5-DIETHYLAMINOPHENOL BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The use of 6-methyl-2-benzothiazolylazo)-5-diethylaminophenol as a pre column derivatizing reagent in the reversed-phase high performance liq uid chromatographic separation and determination of Ru(III), Rh(III), Os(IV), Ir(IV), Pt(II), Co(II), Ni(II) and Cu(II) is reported. When th e mobile phase consists of methanol-water (76/24% v/v) and 20 mmol/l ( pH 5.0) acetate buffer, the eight complexes can be separated within 35 min on a C-8 column. The detection limits are Ru 7.0, Rh 5.1, Os 1.5, Ir 7.6, Pt 3.7, Co 0.62, Ni 0.14 and Cu 1.2 ng/ml, respectively, at a signal-to-noise ratio of 3. RSDs were typically Ca. 1%.