GENE-MAPPING BY CHROMOSOME MICRODISSECTION AND MICROISOLATION IN THE CHICKEN

A chromosome microdissection and microisolation technique in combinati on with filter hybridization was developed for chromosomal localizatio n of cloned chicken genes. The DNA was obtained from microdissected ch romosome regions of metaphase spreads. Dissected DNA was amplified by polymerase chain reaction (PCR). The chicken MHC gene located on the n ucleolar chromosome and beta-actin gene located on chromosome 2q were chosen as tests for the procedure and then detected by dot blot analys is using amplified chromosomal DNA probed with biotinylated DNA. The s tudy establishes the technique of using chromosome microdissection and microisolation for localization of cloned genes as a complementary or alternative approach to both in situ DNA/chromosome hybridization and fluorescent in situ hybridization.