Microelectrodes consisting of a vertical structure made by the superpo
sition of niobium, titanium and gold layers have been fabricated using
electron beam lithography and thin film technique. The niobium layer
improves the mechanical strength and ensures a good resistance of the
structure to the galvanic corrosion which is a very important characte
ristic when dealing with biological particles. The proximity correctio
ns have been applied in order to obtain the PMMA resist with a suitabl
e profile for the lift-off of the Ti/Au layers. This corrections allow
ed us to setup the process to obtain electrode gaps down to 0.2 mu m.