Rj. Hron et al., QUICK METHOD FOR ESTIMATING FREE GOSSYPOL IN COTTONSEED, MEATS, COLLETS, AND EXTRACTED MEALS, Journal of the American Oil Chemists' Society, 73(2), 1996, pp. 199-202
A method for estimating free gossypol (FC) has been developed that dec
reases sample-determination time from over 2 h to about 25 min per sam
ple. With auto pipetters and bottle-top dispensers, six sample determi
nations can be completed in approximately 50 min. The method consisted
of adding water and acetone separately to a fixed sample weight, mixi
ng, filtering, diluting with 65% acetone, and reading absorbance on a
spectrophotometer. Absorbance was plotted against the official America
n Oil Chemists' Society's FC; method for samples that contained FC bet
ween 0.02 and 0.9%. Quadratic least squares regression for 31 samples
had a correlation coefficient of r(2) = 0.986 and a standard error of
estimated FG of 0.032%.