Ks. Huang et Cc. Akoh, ENZYMATIC-SYNTHESIS OF STRUCTURED LIPIDS - TRANSESTERIFICATION OF TRIOLEIN AND CAPRYLIC-ACID ETHYL-ESTER, Journal of the American Oil Chemists' Society, 73(2), 1996, pp. 245-250
Structured lipids were successfully synthesized by lipase-catalyzed tr
ansesterification (ester interchange) of caprylic acid ethyl ester and
triolein. The transesterification reaction was carried out in organic
solvent as reaction media. Eight commercially-available lipases (10%
w/w substrates) were screened for their ability to synthesize structur
ed lipid by incubating with 100 mg triolein and 78.0 mg caprylic acid
ethyl ester in 3 mL hexane at 45 degrees C for 24 h. The products were
analyzed by reverse-phase high-performance liquid chromatography with
evaporative light-scattering detector. Immobilized lipase IM60 from R
hizomucor miehei converted most triolein into structured lipids (41.7%
dicapryloolein, 46.0% monobcapryloolein, and 12.3% unreacted triolein
). However, lipase SP435 from Candida antarctica had a higher activity
at higher temperature. The reaction catalyzed by lipase SP435 yielded
62.0% dicapryloolein, 33.5% monocapryloolein, and 4.5% unreacted trio
lein at 55 degrees C. Time course,-incubation media, added water, and
substrate concentration were also investigated in this study. The resu
lts suggest that lipase-catalyzed transesterification of long-chain tr
iglycerides and medium-chain fatty acid ethyl ester is feasible to syn
thesize structured lipids.