BIOSENSORS IN AUTOMATED-ANALYSIS SYSTEMS .2. FRUCTOSE DETERMINATION IN JUICES BY FRUCTOSEDEHYDROGENASE-THICK-FILM-PLATINUM ELECTRODES

Authors
HANKE A EBERHARDT A BILITEWSKI U GALENSA R KUNNECKE W
Citation
A. Hanke et al., BIOSENSORS IN AUTOMATED-ANALYSIS SYSTEMS .2. FRUCTOSE DETERMINATION IN JUICES BY FRUCTOSEDEHYDROGENASE-THICK-FILM-PLATINUM ELECTRODES, Deutsche Lebensmittel-Rundschau, 92(2), 1996, pp. 35-39
Citations number
15
Categorie Soggetti
Food Science & Tenology
Journal title
Deutsche Lebensmittel-Rundschau → ACNP
ISSN journal
00120413
Volume
92
Issue
2
Year of publication
1996
Pages
35 - 39
Database
ISI
SICI code
0012-0413(1996)92:2<35:BIAS.F>2.0.ZU;2-C
Abstract
An amperometric fructose-sensor was produced by immobilization of fruc tosedehydrogenase on the working electrode of thick-film platinum elec trodes. Potassium ferricyanide served as mediator for electron transfe r. The sensor was tested as a detector in flow injection analysis (FIA ), flow diffusion analysis (FDA) and HPLC. For the analysis of juices, the coupling of the enzyme electrode with HPLC showed the best agreem ent with the results of common enzymatic fructose determination and tu rned out to be a suitable method for laboratory analysis. However it i s beaten by FIA concerning the achievable frequency of analyses.