Bn. Okolo et Li. Ezeogu, ENHANCEMENT OF AMYLOLYTIC POTENTIAL OF SORGHUM MALTS BY ALKALINE STEEP TREATMENT, Journal of the Institute of Brewing, 102(2), 1996, pp. 79-85
The effect of alkaline steep liquor on sorghum maltability was investi
gated using three improved Nigeria sorghum cultivars, Germination was
for four days at 30 degrees C after steeping under four different regi
mes, Grain germinability, root length and malting loss were significan
tly (P < 0.001) repressed by steeping in alkaline liquor for all the c
ultivars, However, the extent of this repression seemed in all cases s
ignificantly dependent on cultivar and steep regime plus their possibl
e interactions, Similarly, the development of diastatic enzymes activi
ties appeared to be highly significantly dependent on steep liquor, st
eep regime, cultivar, plus their possible pairwise and three way inter
actions, For ICSV400, highest diastatic power and a-amylase developmen
t were attained on steeping grains in alkaline liquor under a continuo
us steep regime incorporating final warm steep treatment. Conversely,
exposure of KSV8 and SK5912 to a regime incorporating air-resting and
final warm steep significantly enhanced diastatic power and alpha-amyl
ase development, beta-Amylase activity was in all cases enhanced by al
kaline steeping, In fact, beta-Amylolytic activity constituted over 70
% of total diastatic activity in most alkaline steeped ICSV400 malts,
However, SK5912 exhibited relatively low hot water extract in spite of
the improved amylolytic activity.