Mj. Facon et Bj. Skura, ANTIBACTERIAL ACTIVITY OF LACTOFERRICIN, LYSOZYME AND EDTA AGAINST SALMONELLA-ENTERITIDIS, International dairy journal, 6(3), 1996, pp. 303-313
The antibacterial activity of a pepsin digest of bovine lactoferrin, c
ontaining the peptide lactoferricin B against Salmonella enteritidis w
as studied under various conditions. The digest had greater antibacter
ial activity than lactoferrin in 1% peptone, was bactericidal and also
increased the permeability of the outer membrane of the bacteria as d
emonstrated by plating on bile salts agar. The antibacterial activity
was inhibited in richer media, such as tryptic soy broth or milk-based
or soy-based infant formula. Addition of lysozyme or EDTA or both, to
the lactoferrin digest resulted in increased antibacterial activity o
f the mixture. Lysosyme or EDTA alone had little or no activity agains
t S. enteritidis. The increase in antibacterial activity obtained with
the addition of lysozyme and EDTA to the lactoferrin digest was not s
ufficient to overcome inhibition by components of the richer media.