L. Mansfield et S. Forsythe, COLLABORATIVE RING-TRIAL OF DYNABEADS(R) ANTI-SALMONELLA FOR IMMUNOMAGNETIC SEPARATION OF STRESSED SALMONELLA CELLS FROM HERBS AND SPICES, International journal of food microbiology, 29(1), 1996, pp. 41-47
Eight laboratories participated in a Salmonella detection ring-trial w
hich compared selective enrichment by conventional broths with immunom
agnetic separation (IMS) using Dynabeads(R) Anti-Salmonella. Laborator
ies analyzed six types of herbs and spices that were spiked with one o
f six freeze-dried Salmonella species. Each herb and spice analysis co
mprised of 12 samples (25 g each) which had been spiked at three diffe
rent levels, plus a negative control and stored for one week prior to
testing. Out of a total 468 samples analyzed, 195 (41.7%) were positiv
e by both methods. Eighteen samples were positive only by IMS enrichme
nt, in comparison with 19 positive samples by conventional enrichment
broths and not IMS. These results confirm the potential use of IMS as
an alternative to enrichment broths for Salmonella isolation.