Sl. Abidi et al., A PREFERRED SOLVENT SYSTEM FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SOYBEAN PHOSPHOLIPIDS WITH EVAPORATIVE LIGHT-SCATTERING DETECTION, Journal of the American Oil Chemists' Society, 73(4), 1996, pp. 535-536
Normal-phase high-performance liquid chromatography separations of pho
spholipids (PL) depend heavily on the variation in mobile phases. Inco
rporation of pure tetrahydrofuran to a mobile phase of chloroform, met
hanol, and ammonium hydroxide improved the separation of polar phospho
lipids. However, impurities were often found to be present in tetrahyd
rofuran that caused severe peak interferences with PL analytes. The us
e of a common chromatographic solvent tert.-butyl methyl ether, in lie
u of tetrahydrofuran in mobile phases, eliminated problems associated
with solvent contamination and yielded reproducible results. The modif
ied method has been applied for the quantitative analysis of phospholi
pids in crude soybean oils.