W. Fritzsche et al., RECONSTRUCTION OF RIBOSOMAL-SUBUNITS AND RDNA CHROMATIN IMAGED BY SCANNING FORCE MICROSCOPY, Journal of vacuum science & technology. B, Microelectronics and nanometer structures processing, measurement and phenomena, 14(2), 1996, pp. 1405-1409
Scanning force microscopy (SFM) reveals surface topography by scanning
a sharp tip in dose proximity to the sample. Due to tip-sample intera
ction, artificial broadening of the real surface structure with the ti
p geometry occurs. One approach for image reconstruction is the use of
calibration standards, preferably in the size range of the samples. I
n the present study, an image reconstruction method based on colloidal
gold as a geometric standard was used to reconstruct SFM images of bi
omolecules. Sample and calibration standard size were in the nanometer
range, and the standards were coadsorbed with the specimen. Raw and r
econstructed images of the biomolecules were compared, and the reconst
ruction was characterized by difference images as well as determinatio
n of the difference volume. The application of image reconstruction ba
sed on colloidal gold as a calibration standard for SFM of biomolecule
s is discussed. (C) 1996 American Vacuum Society.