Tl. Mounts et al., EFFECT OF GENETIC-MODIFICATION ON THE CONTENT AND COMPOSITION OF BIOACTIVE CONSTITUENTS IN SOYBEAN OIL, Journal of the American Oil Chemists' Society, 73(5), 1996, pp. 581-586
The content and composition of tocopherols, sterols, and phospholipids
in soybean oils derived from genetically-modified soybeans were deter
mined by normal and reverse-phase high-performance liquid chromatograp
hy and gas-liquid chromatography. Tocopherol content was lowered in oi
ls from soybeans selected to yield high palmitate and stearate content
s. However, beta-tocopherol, which amounts to less than 1 ppm in contr
ol oils, was increased to 25-53 ppm in these oils. Sterol content was
higher in one reduced-linolenate oil, which also had the highest oleat
e content. The greatest variability was observed in the content of bet
a-sitosterol, which ranged from 46.9-151.6 mg/100/g in the modified oi
ls. Although, in general, there was little impact on the phospholipids
, the content of phosphatidic acid was elevated in crude oils from thr
ee of the lines. Increases in phosphatidic acid are generally associat
ed with storage deterioration of soybeans. Individual major classes of
phospholipid were isolated, and the molecular species composition of
each was determined. Compositional variations in molecular species ind
icated that there was an impact of the genetic modification of soybean
s at the molecular level of the phospholipids that are primary plant c
ell components.