KINETIC EVALUATION OF 3-BETA-HYDROXYCHOLEST-5-EN-7-ONE (7-KETOCHOLESTEROL) STABILITY DURING SAPONIFICATION

To clarify conflicting information regarding 7-ketocholesterol (7-KC) recovery from saponification, we evaluated the stability of 7-KC in me thanolic alkaline medium. We added 1 N alcoholic KOH to 7-KC or lard s piked with 7-KC and held the mixtures at 45, 55, 65, and 75 degrees C for different times to simulate various saponification conditions. Gas -chromatographic determination of residual 7-KC with 5 alpha-cholestan e as the internal standard (IS) showed that the higher the saponificat ion temperature, the greater the 7-KC degradation. Hot saponification at 75 degrees C for 30 min caused extensive destruction and left only 31% 7-KC. 7-KC loss during saponification could be described by pseudo first-order kinetics, and the dependence of degradation rate on tempe rature (T, K) by k (h(-1)) = (2.6 x 10(17)) exp (-1.36 x 10(4)/T). As predicted by the kinetic equation, 7-KC loss during room-temperature s aponification (21 degrees C) was practically negligible; following the exposure of 7-KC or lard spiked with 7-KC to 1 N alcoholic KOH for 18 h, about 97% 7-KC was recovered. 6-Ketocholestanol, when used as an I S, should be looked at carefully because of potential tautomerization, leading to the formation of two enol isomers when in extended contact with trimethylsilyl derivatization reagents.