Bf. Lin et al., DIETARY-FAT INFLUENCES IA-ANTIGEN EXPRESSION, CYTOKINES AND PROSTAGLANDIN E(2) PRODUCTION OF IMMUNE CELLS IN AUTOIMMUNE-PRONE NZBXNZW F1-MICE, British Journal of Nutrition, 75(5), 1996, pp. 711-722
To elucidate further the influences of dietary fat on autoimmune disea
ses, two groups of NZB/W F1 mice were fed with diets containing 200 g
dietary fat/kg and 50 g dietary fat/kg (control) respectively, The dif
ference in energy intake between these two groups was compensated with
carbohydrate, Mice were bled regularly every month and some of them w
ere killed for in vitro experiments after 5 months experimental diets,
Higher immunoglobulin (Ig)M and IgG anti-double stranded DNA antibody
levels, shortened life span and worsened proteinuria were noted in mi
ce fed on the high-fat diet compared with those fed on 50 g dietary fa
t/kg, Phenotypic analyses of spleen cells and peritoneal exudate cells
showed that the percentage of CD5(+) B cells and the mean fluorescent
intensity of major histocompatibility molecules on the surface of bot
h types of cells were higher in mice fed on the high-fat diet, In gene
ral, higher type 2 T-helper cell activity,vas noted in mice fed on the
high-fat diet, In addition, cytokines such as interleukin-6, tumour n
ecrosis factor-cc and prostaglandin E(2) (PGE(2)) produced by lipopoly
saccharide-stimulated peritoneal exudate cells were also higher in the
high-dietary-fat group, These studies suggest that high dietary fat a
nd its related PGE(2) level might have a critical effect on the freque
ncy of CD5(+) B cells, cytokine production, macrophage function and su
bsequent autoimmune regulation in autoimmune mice.