IMPROVED NARP-HPLC METHOD FOR SEPARATING TRIGLYCERIDES OF PALM OLEIN AND ITS SOLID FRACTIONS OBTAINED AT LOW-TEMPERATURE STORAGE

Non-aqueous reversed-phase high-performance liquid chromatography (NAR P-HPLC) with refractive index (RI) detection is described and used for palm olein and its fractions obtained at 12.5 degrees C for 12-24 h. The calculation formula for fatty acid methyl esters (FAMEs) and carbo n number (CN) from the data obtained by NARP-HPLC is described and cor rection factors for all carbon numbers and fatty acids are tabulated. The results were compared with those obtained from FAMEs analysed on 1 0% SP 2330 Supelco packed column gas-liquid chromatography (GLC) and C N analysed on 3% OV-1 Supelco packed column GLC. The results were foun d to agree well for C48, C50, C52, C16:0, C18:0 and C18:1 (correction factor approximate to 1.0); however, a slight variation was observed f or components C54, C14:0 and C18:2 (correction factor 1.0+/-0.37). Cop yright (C) 1996 Elsevier Science Ltd