Pz. Swe et al., IMPROVED NARP-HPLC METHOD FOR SEPARATING TRIGLYCERIDES OF PALM OLEIN AND ITS SOLID FRACTIONS OBTAINED AT LOW-TEMPERATURE STORAGE, Food chemistry, 56(2), 1996, pp. 181-186
Non-aqueous reversed-phase high-performance liquid chromatography (NAR
P-HPLC) with refractive index (RI) detection is described and used for
palm olein and its fractions obtained at 12.5 degrees C for 12-24 h.
The calculation formula for fatty acid methyl esters (FAMEs) and carbo
n number (CN) from the data obtained by NARP-HPLC is described and cor
rection factors for all carbon numbers and fatty acids are tabulated.
The results were compared with those obtained from FAMEs analysed on 1
0% SP 2330 Supelco packed column gas-liquid chromatography (GLC) and C
N analysed on 3% OV-1 Supelco packed column GLC. The results were foun
d to agree well for C48, C50, C52, C16:0, C18:0 and C18:1 (correction
factor approximate to 1.0); however, a slight variation was observed f
or components C54, C14:0 and C18:2 (correction factor 1.0+/-0.37). Cop
yright (C) 1996 Elsevier Science Ltd