Mhp. Moers et al., DETECTION OF FLUORESCENCE IN-SITU HYBRIDIZATION ON HUMAN METAPHASE CHROMOSOMES BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY, Ultramicroscopy, 61(1-4), 1995, pp. 279-283
Fluorescence in situ hybridization signals on human metaphase chromoso
mes are detected by a near-field scanning optical microscope. This mak
es it possible to localize and identify several fluorescently labeled
genomic DNA fragments on a single chromosome with a resolution superio
r to traditional fluorescence microscopy. Several nucleic acid probes
have been used. The hybridization signals are well resolved in the nea
r-field fluorescence images, and the exact location of the probes can
be correlated to the topography as it is afforded by the shear-force f
eedback.