O. Mellenthin et R. Galensa, DETECTION OF SOY IN MEAT-PRODUCTS BY USIN G CAPILLARY ZONE ELECTROPHORESIS AND PHOTODIODE-ARRAY DETECTION, Deutsche Lebensmittel-Rundschau, 92(6), 1996, pp. 171-175
The amount of four selected isoflavonoids present were examined in soy
and meat products with the addition of soy using capillary zone elect
rophoresis and photodiode array detection. The method permits a sensit
ive and quick qualitative detection of soy in meat products. It is bas
ed not on immunological protein differentiation as conventional method
s are, but rather on the identification of the naturally occuring cont
ents in the soy derived from the secondary metabolism of the plants. D
ifferent soy products were examined for their isoflavonoid pattern. Th
e isoflavonoids Genistein and Daidzein and their glycosides were used
as indicating substances, These could clearly be detected in industria
lly manufactured sausages with soy contents varying a from 1% to 6%. E
ven below amounts of 2% an assured qualitative detection of soy additi
ves, especially that of the frequently used soyisolates, was possible.
The experiment included samples of different fat contents and differe
nt heating processes. High manufacturing temperatures didn't interfere
with the measurement. Until now only qualitative and semiquantitative
results could be obtained with assurance.