A NOVEL-APPROACH TO THE SOLUBILITY MEASUREMENT OF PROTEIN CRYSTALS BY2-BEAM INTERFEROMETRY

Authors
SAZAKI G KURIHARA K NAKADA T MIYASHITA S KOMATSU H
Citation
G. Sazaki et al., A NOVEL-APPROACH TO THE SOLUBILITY MEASUREMENT OF PROTEIN CRYSTALS BY2-BEAM INTERFEROMETRY, Journal of crystal growth, 169(2), 1996, pp. 355-360
Citations number
16
Categorie Soggetti
Crystallography
Journal title
Journal of crystal growth → ACNP
ISSN journal
00220248
Volume
169
Issue
2
Year of publication
1996
Pages
355 - 360
Database
ISI
SICI code
0022-0248(1996)169:2<355:ANTTSM>2.0.ZU;2-8
Abstract
A novel technique was developed for the rapid solubility measurement o f protein crystals (lysozyme) by two-beam interferometry. The merits o f this technique are: (1) time saving (less than 2 h to obtain one exp erimental point), (2) the small amount of the sample (similar to 66 mu l), (3) high accuracy, (4) applicable to the solubility measurement o f a metastable phase and (5) insensitive to impurities. The limitation s of the present technique are also discussed.