SEPARATION AND IDENTIFICATION OF XIMENYNIC ACID ISOMERS IN THE SEED OIL OF SANTALUM-SPICATUM RBR AS THEIR 4,4-DIMETHYLOXAZOLINE DERIVATIVES

The seed oil of Santalum spicatum contains a significant amount of xim enynic acid, trans-11-octadecen-9-ynoic acid, a long-chain acetylenic fatty acid, as a major component (34%). The identity of trans-ximenyni c acid was confirmed after isolation by ultraviolet, infrared, and nuc lear magnetic resonance (NMR) (H-1- and C-13-) spectroscopy and by gas chromatography/mass spectrometry (GC/MS). The cis isomer of ximenynic acid was also found (< 1%) in some samples. The cis and trans isomers were characterized by GC/MS comparison of their methyl esters and 4,4 -dimethyloxazoline derivatives.