GENERATION AND APPLICATION OF IMMUNOLOGICAL PROBES FOR THE STUDY OF MEMBRANE-BOUND PROTEINS AND ENZYMES - A REVIEW

Immunological techniques are widely used for the detection of enzymes in food systems and for elucidating protein structure and function. Th e generation of antibodies recognizing membrane-bound proteins is diff icult due to their hydrophobic nature, and reduced antigenicity. This review summarizes strategies for the production and purification of po lyclonal antibodies recognizing membrane proteins. Applications such a s enzyme immunoprecipitation, use of site-specific anti-peptide antibo dies for determination of the topology of membrane-embedded proteins, immunocytochemistry, and expression vector cloning are described.