2,4,5-TRIHYDROXYBUTYROPHENONE (2,4,5-THBP) AS A SUBSTRATE FOR HORSERADISH-PEROXIDASE

Authors
KAHN V ZAKIN V
Citation
V. Kahn et V. Zakin, 2,4,5-TRIHYDROXYBUTYROPHENONE (2,4,5-THBP) AS A SUBSTRATE FOR HORSERADISH-PEROXIDASE, Journal of food biochemistry, 20(2), 1996, pp. 93-109
Citations number
20
Categorie Soggetti
Biology,"Nutrition & Dietetics
Journal title
Journal of food biochemistry → ACNP
ISSN journal
01458884
Volume
20
Issue
2
Year of publication
1996
Pages
93 - 109
Database
ISI
SICI code
0145-8884(1996)20:2<93:2(AASF>2.0.ZU;2-K
Abstract
2,4,5-trihydroxybutyrophenone (2,4,5-THBP), in the presence of hydroge n peroxide (H2O2), is a substrate for horseradish peroxidase (HRP) wit h a Km value of 2.5 mM. An intermediate red product(s), probably 2,4,5 -THBP quinone, characterized by a peak at 490-500 nm, was detected and the time course of its conversion to the final red product(s) was stu died. The relationships between the rate of 2,4,5-THBP oxidation to pi gmented product(s) as a function of various concentrations of H2O2, 2, 4,5-THBP and HPR are described.